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To determine the diversity of macroinvertebrates and their importance in the freshwater ecosystem of river Seco, the river was divided into a buffer area (sites 1 and 2) and an area of direct impact (site 3), monitored between July and September 2019. Samples were taken from leaf litter, stones, and logs at the riverbanks or submerged in the water, and a Surber net was also used. The captured specimens were transported to the laboratory in airtight bags and identified using taxonomic keys. A total of 706 macroinvertebrate specimens were collected, distributed across 10 orders and 28 families. The most abundant orders were Ephemeroptera (262), Coleoptera (167), Hemiptera (120), Diptera (71), and Trichoptera (57). To determine diversity (H'), equitability (J'), and dominance (D'), the Shannon-Wiener, Pielou, and Simpson indexes were used. The results showed medium diversity, a good mix of species, with a relatively equitable distribution among them, which is favorable for the biodiversity of the ecosystem. The SVAP protocol yielded a result of 8.27, indicating that the studied area of río Seco has good quality.
